习岗, 赵燕燕, 刘锴, 贺瑞瑞. 蛋白质合成抑制剂对萌发玉米超弱光子辐射的影响[J]. 农业工程学报, 2015, 31(10): 303-308. DOI: 10.11975/j.issn.1002-6819.2015.10.041
    引用本文: 习岗, 赵燕燕, 刘锴, 贺瑞瑞. 蛋白质合成抑制剂对萌发玉米超弱光子辐射的影响[J]. 农业工程学报, 2015, 31(10): 303-308. DOI: 10.11975/j.issn.1002-6819.2015.10.041
    Xi Gang, Zhao Yanyan, Liu Kai, He Ruirui. Effect of protein synthesis inhibitors on ultraweak photon emission during germination of corn[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2015, 31(10): 303-308. DOI: 10.11975/j.issn.1002-6819.2015.10.041
    Citation: Xi Gang, Zhao Yanyan, Liu Kai, He Ruirui. Effect of protein synthesis inhibitors on ultraweak photon emission during germination of corn[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2015, 31(10): 303-308. DOI: 10.11975/j.issn.1002-6819.2015.10.041

    蛋白质合成抑制剂对萌发玉米超弱光子辐射的影响

    Effect of protein synthesis inhibitors on ultraweak photon emission during germination of corn

    • 摘要: 为了揭示种子萌发过程中超弱光子辐射机理,分别采用蛋白质合成的转录抑制剂放线菌素D(actinomyin D,AMD)和翻译抑制剂环己亚胺酮(cycloheximide, CHM)处理萌发玉米种子,研究了玉米萌发过程中鲜质量的变化以及自发光子辐射和外界光诱导的延迟光子辐射的变化。结果表明,50 μg/mL的AMD部分抑制了萌发玉米鲜质量的增长,100 μg/mL的CHM完全抑制了萌发玉米鲜质量的增长,萌发玉米自发光子辐射强度的增长与种子鲜质量的增长呈现正相关(相关系数r分别为0.95492、0.93218和0.96235)。研究还发现,在玉米萌发过程中,AMD和CHM对延迟光子辐射的增长有不同的抑制作用,CHM部分抑制了延迟光子辐射的初始光子数、相干时间和积分强度的增大,AMD则使初始光子数、相干时间和积分强度不再增加。研究结果为揭示种子萌发过程中超弱光子辐射的机理及其技术开发提供参考。

       

      Abstract: Ultraweak photon emission in germination of plant seeds which includes spontaneous photon emission and delayed photon emission induced by external light is a kind of life information. Spontaneous photon emission is closely related to important life processes such as DNA synthesis, cell division, respiratory metabolism and biological oxidation. Delayed photon emission is related to functional status of biological systems. The study of ultraweak photon emission in germination of plant seeds has very important significance in the mechanism of seed germination, resistance evaluation, identification of germplasm and so on. In order to reveal the ultraweak photon emissions mechanism in seed germination process, the transcription inhibitor of protein synthesis i.e. actinomyin D (AMD) and translation inhibitor i.e. cycloheximide (CHM) were used to treat germinating corn seed respectively. The changes of the fresh quality, spontaneous photon emission and delayed photon emission of germinating corn were studied in this paper. The results showed that the 50 μg/mL AMD partly inhibited the increase of fresh quality during the germination of corn, 100 μg/mL CHM completely inhibited the increase of fresh quality of germinating corn, and there was a positive correlation between the spontaneous photon emission and seed's fresh quality during the germination of corn (correlation coefficient was 0.96235). It was implied that the intensity of spontaneous photon emission from germinating corn could be used as a signal for indicating the germinating status of corn. The study also found that AMD and CHM had different inhibitory effects for the increase of delayed photon emission during germination of corn, of which, the inhibition from AMD was stronger than CHM. It was speculated that respiratory metabolism and RNA/DNA synthesis in germinating corn could be the sources of spontaneous photon emission. The further study on delayed photon emission induced by external light found that the initial photon number I0 and coherence time τ of kinetic parameters about delayed photon emission during the germination of corn gradually increased; CHM treatment partially inhibited the increase of I0 and τ, and the inhibition of AMD was greater than CHM. According to biological significance of these kinetic parameters about delayed photon emission, it was suggested that CHM treatment caused the disorder of respiratory metabolic system and decreased the intensity of respiratory metabolism, and AMD treatment caused the disorder of respiratory metabolic system and RNA/DNA synthesis system and completely inhibited the increase in respiration of cells during germination of corn. The area under the curve of delayed photon emission was called delayed photon emission integral intensity, represented by I(T). The study in this paper showed that the delayed photon emission integral intensity I(T) gradually increased in corn germination process, CHM treatment inhibited the increase of I(T), AMD treatment completely inhibited the increase of I(T), and the inhibition of CHM and AMD on I(T) was the same as that on I0. This result further illustrated that the RNA/DNA synthesis reactions and respiratory metabolic system could be the sources of delayed photon emission from germinating corn seeds. The size of delayed photon emission integral intensity I(T) could be expressed as the strength of the respiratory metabolism and RNA/DNA synthesis reactions during the germination of corn seeds. These research results provide a reference for understanding the mechanism of ultraweak photon emissions from germination seed and for corresponding technology development.

       

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