Abstract:
Abstract: Physical agricultural technique that plant seeds are treated by high voltage electric field (HVEF) is a kind of simple method and has characteristics of high efficiency, short processing time, low power and no pollution, etc, which has become a hot research subject in agriculture field. However, the researches of sorghum seeds treated with HVEF haven't been under way. Some researches suggest that the features of biotic effects of HVEF on plant seeds mainly include not equivalent dose, pluralism of the parameter, stages, extinction effect, pleiotropy and threshold effects. Suitable treatment of HVEF is beneficial to the synthesis of adenosine triphosphate (ATP) and the activation of enzymes in seeds, at the same time, it can strengthen the ability of resisting adversity and increase germination activity of the seeds. To understand the effects of different HVEF treatments on the sorghum seeds germination vigor, using Jinza 122 (sorghum seeds) as the testing subject, and using the integrated method combining germination test, biochemical analysis and quadratic general rotary unitized design, the effects of electric field strength and treatment time on the vigor index and the content of endogenous gibberellin (GA) of Jinza 122 were investigated in this paper; and the optimum plan of HVEF treatment was sought by establishing quadric polynomial egression model which was based on the value of objective function of the vigor index and the content of endogenous GA. The results showed that the effects of different treatments of HVEF on the vigor index and the content of endogenous GA of Jinza 122 had significant difference (P<0.05); analysis of main effect indicated that the effects of electric field strength were more than that of treatment time on the vigor index and the content of endogenous GA of Jinza 122; single effect analysis indicated that the influence of electric field strength and treatment time on the vigor index and the content of endogenous GA of Jinza 122 had thresholds; the interactive influence of electric field strength and treatment time on the vigor index and the content of endogenous GA of Jinza 122 presented respectively a negative and a positive effect which was significant (P<0.05), and the interactive influence of electric field strength and treatment time on the content of endogenous GA was more than on the vigor index of Jinza 122; after optimizing the model, the optimization scheme which should be met simultaneously that the vigor index and mass fraction of endogenous GA of Jinza 122 were greater than or equal 14.25 and 11.55 ng/g was that electric field strength was 485.53-605.38 kV/m, and treatment time was 31.41-48.99 min. On this basis, further optimized conditions were determined as follows: electric field strength was 500 kV/m, and treatment time was 36 min, It had been verified by experiment that the theoretical values of the vigor index and mass fraction of endogenous GA of Jinza 122 which were 15.50 and 14.92 ng/g were consistent with the measured values which were (15.38±0.18) and (14.85±0.13)ng/g. It was further verified the reliability of the scheme. The results presented provide the theoretical basis for the application of HEVF technology in processing seeds in the agricultural production.