Abstract: Abstract: To overcome the shortages of the existing methods for skeleton lines detection such as low adaptability and meet the needs of recognition for navigation path in modern precision spraying technology system, a new algorithm of skeleton lines detection was proposed based on maximum square principle in this paper. In the first part, pretreatment operation was applied to process the corn crop rows image. Firstly, the improved super green gray transformation algorithm (1.68G-R-B) was used to transfer the corn crop rows color image into gray-scale image and the corn crop rows was separated from the background for the first time. Compared with the traditional gray-scale methods, the improved algorithm in this article not only distinguished the crop rows and background better but also greatly reduced the noise interference and the processing time. Secondly, in order to split the crop rows more clearly, the middle filter operation was used to eliminate background noise. Thirdly, threshold segmentation method was used to convert gray-scale image into a binary image so to prominent the crop rows area further and extinction the background area, and the crop rows and the background were completely separated by the threshold segmentation. In the second part, the corrosion and expansion operation of morphological algorithm were used to process the above binary image. The 3×3 template element of corrosion was selected to eliminate the background noise that was smaller than the area of crop rows after binarization. The 5×1 template elements of expansion were selected to connect the discontinuous area goodly. In order to get the best contour of the corn crop rows, the times of corrosion and expansion operation was determined by experiment. In the third part, the skeleton of corn crop rows was extracted by maximum square principle that was put forward by this paper. Firstly, the region of crop rows was divided base on symmetry. Secondly, the number of pixels that the value was one in the maximum square of the undetermined skeleton points in each region was written. Finally, comparison of the numbers in each row and the undetermined skeleton points was made so that the one with the most value was selected as target skeleton points. In order to evaluate the advantage of the algorithm, maximum square frame extraction algorithm was compared respectively with morphological skeleton extraction and maximum disk skeleton extraction algorithm which is used extensively by researchers. At the same time, the skeleton line of central crop rows were extracted and linear fitting operation was carried out to verify the accuracy of the algorithm. The random Hough transform was used to get the navigation line because of its advantage. The deviation between the center line of crop rows were fitted and actual navigation line was used to determine the accuracy of skeleton extraction. Image of other crop rows was used to prove the adaptability of the algorithm. Experimental results showed that the new algorithm could not only maintain a single pixel and has strong anti-interference ability of edge noise but also extract the skeleton lines more accurately. In addition, it also could be adapted for the skeleton extraction of other crops as well. And the error of skeleton was less than 5 mm and can satisfy the demand of precision spraying.