Abstract: Abstract: Radish is one of the most consumed vegetables，which is also a nitrate-rich root vegetable. Numerous studies have reported that fertilization was one of the major factors influenced nitrate content in vegetables, especially in radish. However, few researches on the influence of fertilization on nitrate accumulation, distribution and assimilation in vegetables have been carried out. Therefore, Experiments of radish cultivation were adopted to comprehensively investigate the effects of continuous application of organic and inorganic fertilizers on the radish yield, the soil nitrate dynamics, and the absorption, distribution and assimilation of nitrates in vegetable tissue. The radish biomass was declined in the order of half chemical fertilizer plus half organic manure(FOM)＞organic manure(OM)＞chemical fertilizer(F)＞no fertilizer(control, CK). Among them, there were no significant differences between FOM and OM treatments. The nitrate concentrations in leaves and roots were decreased following by F＞FOM＞CK＞OM. And the content of crude protein in radish is in the same order with the nitrate concentration in leaves. Considering all these factors, including radish production, the nitrate accumulations in vegetable and soil, the most moderate fertilization was organic manure plus chemical fertilizer. If considering food safety of vegetables nitrate accumulation and environmental effect of soil nitrate, the single application of organic manure was the best after applying for several seasons. The risk of the nitrate accumulation of radish and the nitrate nitrogen residue of soil were followed by F＞FOM＞OM. The nitrate contents in leaves and root both showed a decreasing trend over time. In the vigorous growth stage of leaves, the nitrate contents in the aboveground parts were higher than those in the underground parts. While in the roots growth period, more nitrates were stored in roots. When chemical fertilizer was applied alone (F), higher nitrate content was absorbed by plant, more enriched nitrates were stored in petiole and inner leaves. When cow manure was single fertilized (OM), low nitrate nutrition was offered in the whole growth period, the accumulative nitrate in vegetables tissues were released to the mesophyll and outer leaves to maintain the normal growth of radish. Therefore, continuous application of organic fertilizer, not only decreased the nitrate content in vegetables, but also increased the vegetable yield in the latter seasons.