Abstract:
Abstract: Ganoderma lucidum is a typical polypore fungus used for traditional Chinese medical purposes. The active ingredients of Ganoderma lucidum include polysaccharides, triterpene, proteins and ganoderic acid, which can be effective in the treatment of cancer, hypertension and viral infections. Therefore, it has been widely planted and used in human health products. Nowadays the main cultivation methods for Ganoderma lucidum were cut-log cultivation and substitute cultivation. These two methods use abundant wood. So the cultivation of Ganoderma lucidum by using abundant wood could cause destruction to the ecological environment. Ganoderma lucidum liquid fermentation cultivation was no only an important method for fundamental research, but also a way to product bioactive compounds. In this study, cellulose, hemicellulose and lignin were added into the media to investigate the influence of these additives on the growth of Ganoderma lucidum. The results showed that adding lignin or hemicellulose all could obviously promote the growth of Ganoderma lucidum. However, adding cellulose could not obviously promote the growth of Ganoderma lucidum. Most of lignin and a part of hemicellulose could be used, while the cellulose could not be used for the growth of Ganoderma lucidum. The effects of liquid culture media with different lignin concentrations on the growth of Ganoderma lucidum were studied. The liquid fermentation media with four different lignin concentrations (0.2%, 0.5%, 1.0%, 1.5%) were designed. The results showed that adding little lignin could promote the growth of Ganoderma lucidum, however, when the concentration of lignin was above 1.0%, the growth of Ganoderma lucidum was severely inhibited. Compared with the control, the growth of Ganoderma lucidum was the best with the lignin concentration of 1.0%, and its biomass was increased by 56%; the synthesis of extracellular polysaccharides and intracellular polysaccharides were increased by 33.6%, 43%, respectively; and the synthesis of extracellular triterpenoid and intracellular triterpenoid were increased by 34.6%, 41%, respectively. It was proved that adding lignin in the process of liquid fermentation culture could increase the yield of polysaccharides and triterpenoid in four different Ganoderma lucidum strains (Ganoderma lucidum 203, wild Ganoderma lucidum, Long Quan Ganoderma lucidum, Jin Zhai Ganoderma lucidum. Compared with the control, the polysaccharide contents of four Ganoderma lucidum were increased by 38%-43%, and triterpenoid contents were increased by 30%-38%, and the polysaccharide content of Ganoderma lucidum 203 was higher than the control by 43%, and triterpenoid content of Jin Zhai Ganoderma lucidum was higher than the control by 38%. However, it will need further study to prove that adding lignin between cut-log cultivation or substitute cultivation can promote the growth of Ganoderma lucidum and the synthesis of bioactive compounds.