Abstract: Abstract: The changes of lake water resources record the impacts of climate changes and human activities on the regional hydrological processes in the changing environment. Especially for inland areas with less precipitation, timely and accurate information on changes in lake water resources are recorded. Environmental evolution has an important reference value. With China’s largest desert freshwater lake-Hongjiannao Lake as an example, based on the 3S (RS, GIS, and GPS, which are remote sensing, global position system, geographic information system) technology, lake water surface area change was analyzed, which provided important scientific guidance for the restoration, protection and utilization of ecological and economic functions of Hongjiannao Lake. However, due to the different sources of data, the uneven technology, the discontinuous time series and the generally short time series, as well as the different monitoring phases, the result analysis on the change of water area of Hongjiannao Lake is quite different and systematic study and overall analysis of long time series of the entire river basin are lacking. Fifteen periods of both basic and RS data collected for Hongjiannao Lake, such as DRG, DLG, DOM, remote sensing images, and paper maps, were selected for analysis over a recent 60-year period (from 1957 to 2015), together with field annotation data and relevant attributes. All the data and images above have passed the geometric correction of digital elevation model data from Shaanxi Surveying and Mapping Archives and Geological Information Bureau of State Bureau of Surveying and Mapping, which have good quality. Different data can meet the high-precision registration requirements of water body information extraction in the study area. Among them, NDWI (normalized difference water index) with better extraction effect was used to extract water body information, which could restrain the vegetation soil while enhance the water body information. To analyze characteristics in spatio-temporal variations and identify trends of the change in lake water area over the period, we comprehensively utilized RS and GIS techniques and adopted spatial data processes, extracted and interpreted information, constructed a massive database, and conducted integrated management and analysis. We also explored major causes that triggered spatio-temporal variations of lake water area by combining climate change and anthropogenic factors from the same period. Results indicated that in a recent 60-year period, the area covered by water significantly changed in the study area. During 1957-1976, the water area continuously increased from 37.6 to 55.53 km2, and reached the maximum value over the entire record of study with increased amplitude of 47.69%. The growth rates in northern and southeastern areas of the lake were relatively fast, followed by other regions. During 1977-1999, the overall water area was stable, approximately 50 km2. During 2000-2015, water area showed a decreasing trend year by year, from 50.27 to 31.05 km2 with decreased amplitude of 38.23%, and all regions showed this shrinking trend except waters near Qibusu River in 2005, in which the reverse occurred and the water area slightly increased. In general, over the last 60 years, the revolution process of the water area of Hongjiannao Lake could be divided into 3 phases corresponding to the annual variability of water area: expanding period (1957-1976), stabilizing period (1977-1999), and shrinking period (2000-2015), showing an overall shrinking trend. The area of lakes and waters has experienced a dynamic trend of expansion-stabilization-atrophy in the recent 60 years, and has shown a significant atrophy situation since 1999 and a strong signal of ecological and hydrological deterioration, deserving attention. Trends in both warming and drying climate may be the major factors, which affect lake water area variation in both expanding and stabilizing periods, and the superimposition of these trends with anthropogenic effects (i.e. a water conservation project construction in the upper streams, coal mining, pavement construction around the lake, and water consumption for irrigation) may be the essential factor leading to water area variations during the shrinking period.