Abstract: Virus-free seed potato is the basis for potato production in field. Nutrient solution cultivation technology for virus-free seed potato can maximize the potential of seed multiplication and avoid bacterial infection. It is now deficient in the systematic research on the management of the nutrient solution for the virus-free potato cultivation in the LED (light emitting diode) plant factory, and the effective guidance for the whole growth duration of potato has not been put forward. This research tried to investigate the influence of nutrient solution management on the whole growth and tuberization of virus-free potato plantlets. The virus-free plantlets were acclimatized for 7 d, and then the plantlets with the height of 6-8 cm were transplanted into vermiculite. The light intensity was set at 300 μmol/(m2•s-1) and the daytime and night temperature were (22±1) ℃ and (16±1) ℃ respectively. Three different tests were performed, and in the first test 3 different formulas of nutrient solution were set up: Hoagland nutrition solution (H), mixed nutrition solution (MX) and Japanese garden test nutrition solution (J). In the second test, for 3 stages i.e. seedling stage, tuber formation and expansion period and mature stage, different combinations of nutrition solution concentration were set up: 0.8MX+0.8MX+0.8MX (A), 0.8MX+MX+0.6MX (B) and 0.8MX+1.2MX+0.6MX (C). In the third test, different combinations of nutrition formula and concentration were set up: H+MX+0.6MX (D), 0.8MX+MX+0.6MX (E) and 0.8MX+MX+0.7MX (F). Different nutrient solutions were watered at seedling stage, tuber formation and swelling stage, and mature stage. The results showed that the leaf area of H treatment were significantly higher than MX treatment at seedling stage, indicating that Hoagland nutrient solution had more advantages on the early growth of potato plants. Tuber number per plant, effective tuber number per plant and the ratio of plants whose effective tuber number was more than 3 treated by MX were 3.2, 2.8 and 83.33%, respectively, which were significantly higher than J and H treatment. At tuber formation and swelling stage, 0.8MX caused the plant to grow thinly and 1.2MX inhibited the plant height elongation, and the 1.0MX treatment was beneficial to plant morphological development. At mature stage, tuber number, effective tuber number, tuber mass, effective tuber mass per plant and the ratio of plants whose effective tuber number was more than 3 under the B treatment were 4.0, 3.5, 23.66 g, 23.18 g and 100% respectively, which were significantly higher than A and C. The plant height, shoot dry mass, leaf area and tuber mass of the 0.6MX treatment were higher than the 0.7MX, indicating that the 0.6MX was conducive to the formation of the yield. Tuber mass, and effective tuber mass per plant under the D treatment were both 29.78 g, which were significantly higher than E and F. Taking into account the convenience of management and control in nutrition solution in the plant factory, the reliability in management and the stability of system, the management of H+MX+0.6MX nutrition solution for the production of virus-free potato tubers has more advantages in LED plant factory.