Abstract: Abstract: The health and production status of dairy cows was affected by low temperature in winter in the cold regions in the north of china. If cows are kept in low temperature for a long time, it will lose a lot of heat, the resistance decreases, and the energy utilization efficiency of the diet decreases, the welfare condition decreases. According to the climate character in Heilongjiang province and the design target is not less than -5 ℃ in test cowshed, this experiment analyzed heat transfer coefficient of single-layer shutter and compared the heat transfer coefficient, surface density and thickness of combination of different materials that can be used to make up new composite insulation shutters. This experiment made some kinds of composite insulation layers, and then selected 2 new insulation shutters from those and conducted a field experimental study in a cattle farm in Heilongjiang province. The determined 2 new combined insulation shutters were the white insulation shutter (white polyester cloth + pearl cotton + spraying-bonded wadding + pearl cotton + white polyester cloth, 1.01 W/(m2·℃)) and grey insulation shutter (PE intertexture cloth + spraying-bonded wadding + needled cotton felt + Aluminized PE intertexture cloth, 0.89 W/(m2·℃)). The study choice one cowshed in a farm as tested house. This cowshed was installed the two insulation shutters on east and west sides adopting the symmetrical installation of north and south which spilt the house into two parts. Then, this study choices the other same cowshed with single-layer shutter as control house. The experiment compared the temperature, humidity and illumination of test house and control house. The results showed that there was significant difference in illumination between the 2 new insulation shutters (P<0.05) and no difference in temperature (P>0.05), thus the illumination of shutters is a important factor in practical production. However, the illumination of the 2 new insulation shutters were significantly lower than the single-layer shutter (P<0.05). On the three working conditions that the south and north shutter both opened 25cm, only the south opened 25cm and both sides closed, the average temperature and relative humidity (RH) in tested house were significantly higher than that of control house and outside (P<0.05). The average temperature range in tested house, control house and outside are ?12.45-?16.70 ℃, ?14.38-?18.50 ℃ and ?16.16-?21.56 ℃, respectively, and the average relative humidity range are 88.53%-97.73 %, 82.78%-93.62% and 74.75%-79.24 %, respectively. So, though the new combined insulation shutters have better thermal insulation performance than single-layer shutter, the cowshed is still in the low temperature and high humidity conditions. This paper suggests that in order to ensure the good environmental conditions of the cowshed in winter, we should continue to improve the building envelope at the same time and improve the ventilation system to dehumidifying the air. When we choose the insulation shutter, we should pay attention on its airtight with the wall around. In conclusion, the form of open-ended cowshed with insulation layers should be cautiously adopted in cold areas.