Abstract: In order to improve the airflow condition of vertical ventilation system with fan-pad evaporative cooling system with ceiling in the beef cattle barn applied to the cooling effect and the uniformity of air distribution in the beef cattle occupied zone, as well as to increase the wind speed. This experiment was conducted using computational fluid dynamics (CFD) method on the basis of the measured data to simulate the airflow condition of the vertical ventilation beef cattle barn with fan-pad evaporative cooling system and ceiling. The size of the barn is 52 m×12 m×4.2 m. The lying area of the beef cattle in the barn is along the length direction, and one group is set up at 2-3 intervals, and 8-12 fattened cattle are raised in each group. There were 81 beef cattle which averaged 330 kg of weight included in the study. In order to increase the wind speed in the animal occupied area without affecting the operation of manual feeding, the plastic ceiling was installed 3.3 m away from the lying area of the beef cattle. However, due to gravity, the middle part of the ceiling was downward, so in the CFD model, the ceiling height was 3.0 m. In the simulation, this experiment improved the original model, the number of cattle has increased to 81 and randomly distributed in the model. Measurement experiments were implemented from July to August 2016, which including test temperature, relative humidity, wind speed, enclosure structure temperature, surface temperature of beef cattle, respiration rate and their body size. The measured heights of wind speed were 0.7, 1.2 and 1.7 m above the floor, and the measured heights of temperature and relative humidity were 0.7 and 1.2 m. Measurements were conducted at 10:00, 12:00, 14:00 and 16:00. The temperature of walls, floors and ceiling were recorded by thermal infrared imager (Fluke Ti400), which were used for modeling. The modeling results showed that average relative error of the wind speed was 27% in Y = 0.7 m, 14% in Y=1.2 m and 13% in Y=1.7 m, respectively. The simulation results showed that vertical ventilation system with fan-pad evaporative cooling system and ceiling in this experiment can improve the uniformity of wind speed and air flow in the house. The environmental test results showed that the air flow in the barn with ceiling was uniform and the wind speed in the beef occupied zone was appropriate, which could provide more suitable environment for beef cattle. The average wind speed of the Y= 0.7 m section was 0.75 m/s, the average wind speed of the Y= 1.2 m section was 0.88 m/s, and the average wind speed of the Y= 1.7 m section was 1.0 m/s. Inner average temperature 30.0 ℃, which was lower than the outside by 16%. The average relative humidity was 80.9% and the concentration of harmful gas was within the standard range, and ETI did not reach the level of heat stress. The results can provide a reference for design of this type of beef cattle house.