Abstract: In order to ensure food safety, a zearalenone(ZEN) detection system was developed based on the measurement of the weak chemiluminescence signal of the reaction between the luminol and hydrogen peroxide(H2O2) catalyzed by horseradish peroxidase (HRP) labeled by ZEN for the food industry. The high-precision photomultiplier tube MD983 with side window and 16-bit AD conversion chip were used to realize accurate measurement of the chemiluminescence intensity signal. Rotating precision turntable was drived by the stepping motor. By optimizing the parameters of S-type pulse drive control curve of the stepping motor, the high-precision positioning control of the turntable was completed, and the test window of the photomultiplier tube and the chemiluminescence hole were accurately aligned. The accurate micro-sampler was driven by a linear guide slide table, and the accurate micro-sample injection of the micron level of the reaction liquid was realized. The competitive immunoassay method was adopted. When the concentration of ZEN was equal to 0 μg/kg, there was a maximum amount of luminescence, which solved the problem of detection accuracy under the condition of low concentration of ZEN. The results showed that the detection limit of the system was 0.1 μg/kg, the standard addition recovery was more than 90%, the determination coefficient of the standard curve was 0.995 6, and The linear range of the system for detecting zearalenone was 0-60 μg/kg. The results show that the Zen detection system can meet the requirements of national food industry for Zen content detection in cereals, and provide a reference for the localization of mycotoxin detection instruments.