Abstract: China has been the largest producer of fruits and vegetables in the world, particularly with an output of over 1 billion tons in 2020. However, the annual loss rate of fruits and vegetables has been up to 15% (only 5% in developed countries). The cold chain can effectively maintain the quality of perishable products for food safety, thereby reducing the process losses under the low-temperature environment. The cold chain environmental monitoring has also been essential to predict the fruit quality, thus regulating the low-temperature environment. Furthermore, ethylene is one of the key monitoring elements to maintain the quality and safety of perishable fruits. However, the existing ethylene monitoring device in a fruit cold chain rarely considers the intelligent interaction with temperature and humidity, leading to low monitoring accuracy and application. Therefore, this research aims to propose an ethylene monitoring calibration model using Extreme Learning Machine (ELM) neural network, and then to verify in a multi-element monitoring device. The temperature, relative humidity, and electrochemical ethylene sensors were integrated to realize the multi-element perception under the cold chain environment. In addition to the inherent voltage signal, the temperature and humidity data of the electrochemical ethylene sensor were introduced as the inputs to construct an ELM neural network ethylene calibration model with a higher learning speed and stronger generalization, thereby enhancing the accuracy and applicability of ethylene online monitoring under a dynamic environment. The ELM neural network ethylene calibration model was integrated into the fruit cold chain environmental multi-element monitoring device, further to calibrate the ethylene sensor data online and verify the environmental multi-element monitoring performance. Meanwhile, a Long Range Radio (LoRa) technology was used to ensure the long-distance, low-cost data transmission in the complete fruit cold chain, including pre-cooling, cold storage, cold chain transportation, and sales trains. The ELM neural network ethylene calibration model was trained at the temperatures of 0, 2, 4, 6, 8 and 12 ℃, compared with the BP model. Further, the developed multi-element monitoring device was used to verify the multi-element monitoring performance in the actual cold storage environment of the fruits. The results showed that the calibration Root Mean Square Error (RMSE) of the ELM neural network ethylene calibration model reached 0.30 μL/L, and the average training time for five training sessions of the ELM model was 0.062 5 s, indicating the better adaptive monitoring performance of ethylene in a dynamic environment. The monitoring RMSE values of temperature element, humidity, and ethylene were 0.46 ℃, 1.65%, and 1.11 μL/L, respectively, fully meeting the actual demand on the accurate monitoring of multi-element for the fruit cold chain environment. The finding can offer a great contribution to accurately conntrolling the cold chain environment, and then predicting the fruit quality, particularly for the effective decision-making on cold chain management.