Abstract: Selenium (Se) is one of the essential trace elements in the process of human nutrient metabolism. The Se deficiency can cause various health risks or disorders of the human body. Fortunately, there are abundant Se-rich soil resources in Ganzhou City of Jiangxi Province, China. It is a high demand to make the Se-rich agricultural products more popular in the markets. A production system of greenhouse vegetables has been one of the most intensive agro-ecosystems in recent years. The facility vegetable industry can be expected to serve as the second-largest pillar industry after Gannan navel oranges, as the facility steel frame greenhouses increased rapidly. However, the Se enrichment in the facility soil is still unclear in southern Jiangxi. It is very necessary to fully utilize the Se soil resources of facilities for the decision-making on the land use management. This study aims to explore the total Se content of greenhouse vegetable soil and the distribution characteristics in Ganzhou City, Jiangxi Province, China. 235 soil samples were collected from the 0-20 cm cultivated layer in the greenhouse vegetable bases in 2021. A geographic information system (GIS) was used to measure the latitude, longitude, and altitude of the soil sampling points. The total Se content was also measured to determine the physicochemical properties in the soil samples. The results showed that the average content of total Se was 0.31 mg/kg in the greenhouse vegetable field in the study area, where the variation range was 0.03 to 1.25 mg/kg. The distribution frequency of soil Se content was followed by the normal distribution. There were the relatively abundant Se-rich soil resources of the greenhouse vegetable plots. Specifically, the total Se content in 85.1% of the soil samples was at or above the level of sufficient Se (>0.175 mg/kg), of which 22.1% of the sample soils belonged to the Se-rich level standard (>0.400-3.000 mg/kg). The Se-enriched soil plots were mainly concentrated in the western and northeastern parts of southern Jiangxi. Only 7.2 % and 7.7 % of the total Se content in the soil samples were at the levels of Se deficiency (>0.125-0.175 mg/kg) and potential Se deficiency (≤0.125 mg/kg), respectively. Furthermore, the Se deficiency plots were distributed in the southwest corner of southern Jiangxi. There was no Se poisoning area. The correlation analysis showed that the total Se content of greenhouse vegetable soil was very significantly negatively correlated with the altitude (P<0.01). The total Se content decreased by 5.35×10-4 mg/kg, as the altitude increased by 1 m. By contrast, the soil total Se content was significantly positively correlated with the latitude and soil pH value. Moreover, the soil's total Se content increased by 0.090 and 0.049 mg/kg, respectively, as the latitude and pH increased by 1° and one unit, respectively. In conclusion, the soil Se resources of greenhouse vegetable fields can be widely expected to serve as the great potential for the promising prospect in southern Jiangxi. A recommendation can be proposed to effectively implement the targeted development strategy of greenhouse Se-enriched vegetables, according to the spatial distribution characteristics of soil total Se content in the study area. The finding can provide a strong reference to promote the high quality and green development of the greenhouse vegetable Se-rich industry in southern Jiangxi Province.