Abstract: Abstract: Vermicomposting is a widely accepted, clean, and sustainable technology to dispose of the solid organic wastes, such as livestock manure, plant straw, garden wastes, and food residues. There is an ever-increasing demand for live earthworms for waste resource utilization in recent years, as the vermicomposting industry grow rapidly. It is necessary to harvest the live earthworm from the large scale vermicompost at the end of vermicomposting. However, the traditional earthworm separation is labor-intensive, costly and time-consuming, depending mostly on manual or simple tools. It is also a high demand to rapidly, high-efficiently separate and harvest the live earthworm from the mature vermicompost, as the vermicomposting scale expands constantly. In this study, a three-step earthworm separation was developed to obtain the optimal harvest parameters and the key kinetic mechanism. The third key step technology named dynamic inclined plane separation was studied in terms of experiment, theory, and simulation. The earthworm separation experiment was carried out to harvest the live earthworm from the resulted vermicompost using Earthworm-Vermicompost Rolling Screen (EVRS) and harvest conveyor. The separation mechanism of dynamic inclined plane was investigated from the kinetic analysis and Electronic Discrete Element Method (EDEM) simulation on the key process. The results indicated that the earthworm dynamic inclined plane separation was effective and feasible. Most adult earthworms and vermicompost were separated and collected independently in the specific harvest area. The optimal parameters were achieved in the earthworm inclined harvest plane for the conical separator in the EVRS, particularly with the conveying velocity of 50 mm/s and the inclined angle of 30°. The earthworm harvest rate was approximately (81.50±5.55)% with almost no vermicompost impurity. Consequently, the harvest system was run the 10 kg mixture in 55.36 s with a total material harvest rate of (96.56±1.79)%. The core separating between the earthworm and vermicompost was attributed to their difference in characteristics and surface properties, resulting in the different force and motion states in the earthworm harvest area. Specifically, the earthworms were carried and moved with the dynamic inclined plane during the key harvest process, due to the wet adhesion behavior. The vermicompost was directly rolled down under the effect of a moving and inclined plane, where the spherical shape led to the decrease in the rolling friction force. A full separation was finally realized, where the earthworm and vermicompost were moved in the opposite direction on the dynamic plane. Therefore, the effective solution can be expected to improve the harvest speed and efficiency of earthworm and vermicompost products. The finding can also greatly contribute to shortening the harvest time and saving the separating expense in the vermicomposting field.