Abstract: Abstract: Sesame seeds is recognized as a good source of lipids and proteins, and its processing is mainly focused on oil production. Sesame is rich in a variety of essential amino acids for human body, which has great development and utilization value. However, the processing of sesame proteins is limited by its poor solubility. Due to the sustainability, economical costs and health benefits of plant proteins, researchers have greatly increased their interest in them. At present, aqueous extraction processing has the capability to realize the comprehensive utilizations of lipids and proteins in sesame. However, the cost of commercial proteases is considerable for large scale applications. Previous studies in our laboratory have confirmed that endogenous proteases in sesame seeds have high activity, and it has great potential for aqueous extraction processing of sesame. While preparing lipid products, endogenous proteases could be used to biologically convert sesame proteins into hydrolysate with high solubility and nutritional function. In addition, in the previous study, the effects of pH value, temperature, and time on proteolytic activity of endogenous proteases in sesame milk were studied, and the proteins were optimally hydrolyzed at pH 4.5 value and 50 ℃ for 6 h catalyzed by the endogenous proteases. In this paper, wet-peeled sesame was dried and pressed in order to obtain the sesame oil and pressed meal. Then, the pressed meal was then hydrolyzed into hydrolysate by endogenous proteases. And the effects of drying temperature on pressed oil yield and proteolytic activity of endogenous proteases in wet-peeled sesame were investigated, which provides reference for the practical applications of endogenous proteases. The results showed that under hydraulic pressing (80 MPa, 1 h), the oil yield of sesame and peeled sesame (50 ℃, 4 h) (the total lipids in sesame as 100%) were 81.76% and 83.57% respectively, indicating that peeling is conducive to the improvement of oil yield. The oil yield of wet-peeled sesame increased to 88.55%-90.98% after drying at 80-180 ℃ for 1 h. For the endogenous proteases, its activity decreased slowly at 80-130 ℃ and remained about 57% at 130 ℃, indicating that endogenous proteases had good thermal stability, then the activity decreased rapidly after 130 ℃ and inactivated at 180 ℃. In order to modify proteins in the pressed meal with endogenous proteases, sesame seeds were dried at 50 ℃ for 4 h to obtain sesame oil and low-denatured pressed meal. After grinding, the sesame milk prepared from pressed meal was incubated at pH 4.5 value and 50 ℃ for 6 h. And hydrolyzed sesame milk was separated into two fractions (skim and precipitation) by centrifugation (3 000 g, 5 min). The results showed that the proteins in the skim still accounted for 66% of total proteins in sesame milk after centrifugation, and the proteins in skim were composed of 38% small peptides (< 1 500 Da) and 37% free amino acids. In addition, there are many bioactive substances in the skim, such as ornithine, serotonin, DL-dopa and so on. The purposes of this study were to evaluate the thermal stability of endogenous proteases in sesame seeds and to develop a new method for production of sesame protein hydrolysate, which enhanced the solubility and bioactive properties of sesame proteins.