Abstract: Low soil aeration often causes the hypoxia stress in the root zone, deteriorating to the normal metabolic activities and growth of crops.Micro-nano oxygenation technology can be expected to serve as the potential type of irrigation water oxygenation. But it is still lacking on the oxygenation effect and the change of dissolved oxygen concentration after entering the soil.In this study, a series of the indoor simulation experiments were carried out to clarify the effect of micro-nano oxygenated water addition on the dissolved oxygen dissipation in soil. Three kinds of oxygen concentration gradient water CK: Tap water (8-9 mg/L), O1:15 mg/L, O2:20 mg/L were prepared to supersaturate the soil moisture in the air-dried and pre-cultivated soil using the micro-nano oxygenation. The oxygen micro-electrode was used to monitor the changes of dissolved oxygen concentration in the soil in real-time. The experiment was also performed on the treatments with the glucose and ammonium sulfate as the carbon and nitrogen substrates in the irrigation water under the different dissolved oxygen content. The variation soil dissolved oxygen dissipation was then determined in each treatment.The results show that: 1) A two-stage law was found in the rapid decline stage, followed by the slow decline stage, according to the increase of soil dissolved oxygen concentration with the time. The dissipation of soil dissolved oxygen was dominated by the gas diffusion in the rapid decline stage. The dissipation curve was conformed to a logarithmic function.In the slow decline stage, the consumption of soil dissolved oxygen was mainly microbial consumption, where the dissipation curve was conformed to the Logistic or linear function.2) In the rapid decline stage of the air-dried soil test, the higher the oxygen concentration was, the longer the high oxygen state in the soil was maintained, and the higher the dissolved oxygen consumption rate was, compared with the conventional water treatment. The retention time of dissolved oxygen in the O1 and O2 treatment was prolonged by 40.11%, and 189.62%, respectively, compared with CK.The rapid decline stage of the pre-cultured soil test also showed a similar law to the air-dried soil test. Compared with the conventional water treatment, the dissolved oxygen dissipation time of O1 and O2 treatment was prolonged by 445.16%, and 2741.94%, respectively.3) Insoilswith low microbial activity(the air-dried soil), oxygen and substrate are the key limiting factors of dissolved oxygen consumption. Both oxygen enrichment and substrate addition could improve the consumption rate of dissolved oxygen in the soil, thus reducing the retention time of dissolved oxygen in the soil. Insoilswith higher microbial activity(the pre-cultivated soil), substrate becomes the key limiting factor of microbial activity, and the high oxygen state in the soil will maintain for a long time after aerobic irrigation. In summary, there was the rapid and then slow decline stage in the change of soil dissolved oxygen content with time after the application of micro-nano oxygenated water. An overall downward trend with first fast and then slow can be expected to effectively enhance the oxygen concentration in the soil for the long period of time.The existence time of available oxygen in the soil can provide the better oxygen environment in the crop roots and soil microorganisms.