Abstract: Granular grain can be stored into the warehouse in food industry. The high loading efficiency is often required to optimize the land use for the effective grain preservation. The crucial warehouse mode has emerged in the regions with limited land resources. The diverse grain varieties can be stored to promote the sustainable development. Currently, the granular grain warehouse normally employs a load-bearing system that constituted by a reinforced concrete frame structure. The vertical gravity load of the grain is supported by the well-beam floors and large-section frame columns, while the lateral pressure exerted by the grain is directly absorbed by reinforced concrete horizontal tie-up beams integrated within the warehouse walls. The structural integrity of granular grain warehouses can also depend on the dynamic lateral pressure exerted by the storage material on the warehouse wall during seismic events. In this study, a scaled-down 1:25 three-story warehouse was designed and then produced to explore the effect of granular grains on the dynamic lateral pressure. Shaking table tests were carried out to simulate the varying ground vibration levels under three seismic waves. The data was collected on the acceleration and the lateral pressure that exerted on its walls. Analysed the seismic response characteristics of the storage material. Furthermore, the overpressure coefficient was obtained from the patterns of dynamic lateral pressure on the walls. The dynamic lateral pressure on the warehouse walls was also calculated. Acorrelation analysis was conducted between the height of warehouse and the timing of the peak moment, when the dynamic lateral pressure was exerted on the warehouse walls. Specifically, there was a noticeable lag in the occurrence of this peak moment, as the height of the building increased. Moreover, the peak moment of dynamic lateral pressure on the warehouse wall was consistently lagged behind that of acceleration on the same wall, particularly with the elevation of the height. The dynamic lateral pressure on the warehouse walls escalated progressively with each ascending floor. In the given floor, this dynamic lateral pressure incrementally intensified along the vertical extent of the warehouse walls. Each floor was found with an average increase of approximately 29% per floor. The dynamic lateral pressure exerted on the upper and middle sections of the warehouse walls was 2.5 and 1.4 times greater than that on the lower sections within the same floor. Consequently, the structural design of granular grain warehouses should consider these variations across different floor levels. The overpressure coefficient of the warehouse wall increased with the elevation of the floor, indicating a higher overall overpressure at upper levels. Within a single floor, the overpressure coefficient peaks at the upper section of the warehouse wall, with the middle and lower sections exhibiting lower and closer. Specifically, the maximum overpressure coefficients were 2.9, 3.4, and 4.1, respectively, for the first, second, and third floors of the warehouse walls. The overpressure on the warehouse walls was of significant concern, when subjected to seismic activity. It is very necessary to consider the influence of the dynamic lateral pressure on the warehouse wall across various floors. The seismic response of the storage materials can be used to elucidate the distribution pattern of dynamic lateral pressure. The overpressure on the warehouse walls can be assessed to calculate the lateral pressure on these walls. The findings can offer a strong reference for the warehouses of granular grains under seismic conditions.