低压静电场处理对蝴蝶兰切花保鲜效果的影响

    Preservation effect of cut Phalaenopsis by Low voltage electrostatic field (LVEF)

    • 摘要: 为探讨低压静电场(low voltage electrostatic field,LVEF)处理对蝴蝶兰切花保鲜效果的影响,该研究以蝴蝶兰切花品种“天堂鸟”为试验对象,探究了低压静电场处理对蝴蝶兰切花瓶插期间观赏品质、理化指标及相关酶活性的变化,并通过低场核磁共振(low-field nuclear magnetic resonance,LF-NMR)技术分析蝴蝶兰切花的水分迁移和水分流失情况。结果表明:瓶插第28天时,与对照组相比,低压静电场组的蝴蝶兰切花能保持较好的外观形态,其鲜质量和花径分别显著(P<0.05)提高了(4.88±0.63)%、(4.98±0.02)%,可溶性糖含量和可溶性蛋白含量分别显著(P<0.05)提高了(0.75±0.11) mg/g、(4.09±0.93)mg/g,相对电导率和丙二醛(malondialdehyde,MDA)含量分别显著(P<0.05)降低了(31.53±0.71) %、(21.96±0.49)nmol/g,超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(catalase,CAT)的活性峰值分别显著(P<0.05)提高了(19.58±0.7)U/g、(20.77±0.24)U/g、(20.21±0.37)U/g,同时结果表明低压静电场处理延缓了蝴蝶兰切花的水分迁移和水分流失现象。综上,低压静电场处理延缓了蝴蝶兰切花的品质劣变,该研究结果可为其他切花的保鲜应用提供一定的理论依据和技术参考。

       

      Abstract: In order to study the effect of Low voltage electrostatic field (LVEF) treatment on the preservation of cut Phalaenopsis, the index changes of cut Phalaenopsis "Paradise Bird" treated by LVEF (test group) and without LVEF treatment (control group) during the vase period was evaluated. These indexes including the fresh weight, flower diameter, soluble sugar content, soluble protein content, relative conductivity, malondialdehyde (MDA) content and related antioxidant enzyme activities (SOD, POD, CAT) of cut Phalaenopsis, and the changes of water migration and water loss of cut Phalaenopsis during the vase period were analyzed in combination with the changes of water phase content of low-field nuclear magnetic resonance technology(LF-NMR), and the water distribution in the cut Phalaenopsis was observed by imaging MRI pseudo-color imaging technology. First, cut Phalaenopsis with good freshness, smooth flower surface and no pests and mechanical damage were selected as the experimental objects, and the cut Phalaenopsis were randomly divided into two groups, the cut Phalaenopsis frozen at low temperature (without LVEF) as the control group and the cut Phalaenopsis treated with LVEF as the testtreated group. Both groups were stored at (4±1) ℃. The results showed that compared with the control group, the cut Phalaenopsis treated by LVEF maintained a better appearance, in which the fresh weight and flower diameter increased by (4.88±0.63) % and (4.98±0.02) %, and the soluble sugar content and soluble protein content increased by (0.75±0.11) mg/g and (4.09±0.93) mg/g, respectively. At the same time, LVEF treatment can maintain the cell membrane permeability of cut Phalaenopsis, slow down the extent of membrane lipid peroxidation, and improve the activity of antioxidant enzymes. Its relative conductivity and MDA content decreased by (31.53±0.71) % and (21.96±0.49) nmol/g respectively. At the same time, LVEF treatment improved the activity of related antioxidant enzymes, with peak levels of SOD, POD and CAT increasing by (19.58±0.7) U/g、(20.77±0.24) U/g and (20.21±0.37) U/g, respectively. When combined with the analysis of water phase state of LF-NMR, the decrease in the peak area ratio of cut Phalaenopsis treated by LVEF was slower compared to the control group, which indicated that the LVEF treatment delayed the change of water migration and water loss of cut Phalaenopsis. Through the analysis of MRI pseudo-color imaging technology, the petals of the control group had shrunk and wilted seriously by 28d. Therefore, LVEF treatment can improve the fresh weight and flower diameter of cut Phalaenopsis, reduce the loss of soluble sugar and soluble protein content during the vase period, delay the increase of relative conductivity and MDA content of cut Phalaenopsis, improve the activity of related antioxidant enzymes, and delay the water migration and water loss of cut Phalaenopsis. The above results showed that the LVEF treatment can effectively maintain the high quality of cut Phalaenopsis, delay its senescence, and improve the ornamental quality of cut Phalaenopsis. The results of this experiment can provide theoretical basis and technical reference for the application of cut Phalaenopsis. At the same time, the LVEF preservation technology is straightforward and easy to operate, cost-effective and effective, which has great potential in commercial utilization and has certain prospects in the cut flower preservation market.

       

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