Abstract:
In order to study the effect of Low voltage electrostatic field (LVEF) treatment on the preservation of cut
Phalaenopsis, the index changes of cut
Phalaenopsis "Paradise Bird" treated by LVEF (test group) and without LVEF treatment (control group) during the vase period was evaluated. These indexes including the fresh weight, flower diameter, soluble sugar content, soluble protein content, relative conductivity, malondialdehyde (MDA) content and related antioxidant enzyme activities (SOD, POD, CAT) of cut
Phalaenopsis, and the changes of water migration and water loss of cut
Phalaenopsis during the vase period were analyzed in combination with the changes of water phase content of low-field nuclear magnetic resonance technology(LF-NMR), and the water distribution in the cut
Phalaenopsis was observed by imaging MRI pseudo-color imaging technology. First, cut
Phalaenopsis with good freshness, smooth flower surface and no pests and mechanical damage were selected as the experimental objects, and the cut
Phalaenopsis were randomly divided into two groups, the cut
Phalaenopsis frozen at low temperature (without LVEF) as the control group and the cut
Phalaenopsis treated with LVEF as the testtreated group. Both groups were stored at (4±1) ℃. The results showed that compared with the control group, the cut
Phalaenopsis treated by LVEF maintained a better appearance, in which the fresh weight and flower diameter increased by (4.88±0.63) % and (4.98±0.02) %, and the soluble sugar content and soluble protein content increased by (0.75±0.11) mg/g and (4.09±0.93) mg/g, respectively. At the same time, LVEF treatment can maintain the cell membrane permeability of cut
Phalaenopsis, slow down the extent of membrane lipid peroxidation, and improve the activity of antioxidant enzymes. Its relative conductivity and MDA content decreased by (31.53±0.71) % and (21.96±0.49) nmol/g respectively. At the same time, LVEF treatment improved the activity of related antioxidant enzymes, with peak levels of SOD, POD and CAT increasing by (19.58±0.7) U/g、(20.77±0.24) U/g and (20.21±0.37) U/g, respectively. When combined with the analysis of water phase state of LF-NMR, the decrease in the peak area ratio of cut
Phalaenopsis treated by LVEF was slower compared to the control group, which indicated that the LVEF treatment delayed the change of water migration and water loss of cut
Phalaenopsis. Through the analysis of MRI pseudo-color imaging technology, the petals of the control group had shrunk and wilted seriously by 28d. Therefore, LVEF treatment can improve the fresh weight and flower diameter of cut
Phalaenopsis, reduce the loss of soluble sugar and soluble protein content during the vase period, delay the increase of relative conductivity and MDA content of cut
Phalaenopsis, improve the activity of related antioxidant enzymes, and delay the water migration and water loss of cut
Phalaenopsis. The above results showed that the LVEF treatment can effectively maintain the high quality of cut
Phalaenopsis, delay its senescence, and improve the ornamental quality of cut
Phalaenopsis. The results of this experiment can provide theoretical basis and technical reference for the application of cut
Phalaenopsis. At the same time, the LVEF preservation technology is straightforward and easy to operate, cost-effective and effective, which has great potential in commercial utilization and has certain prospects in the cut flower preservation market.