Abstract: In order to improve the remediation efficiency of Bidens pilosa L. on farmland heavy metal cadmium (Cd)-contaminated soil, based on pot experiments, in 15 mg/kg Cd-contaminated soil. The effects of three concentrations (25,50 and 75 mg/kg, based on nitrogen) of nitrate nitrogen (NO₃⁻-N), ammonium nitrogen (NH₄⁺-N) and amide nitrogen (amide-N) combined with 1 mmol/L S,S -ethylenediamine disuccinate acid (EDDS) on the growth, Cd accumulation and active oxygen metabolism of Bidens pilosa L. were studied, and the principal component analysis and correlation analysis between different indexes were carried out in order to provide method reference and theoretical basis for the remediation and treatment of typical high concentration Cd contaminated farmland soil. The results showed that the combined treatment of NO₃⁻-N and EDDS significantly improved the growth and Cd accumulation of Bidens pilosa L. in Cd-contaminated soil, compared to NH₄⁺-N or amide-N. Among all treatments, the combined treatment of 75 mg/kg NO₃⁻-N and EDDS significantly promoted the growth of Bidens pilosa L. in Cd-contaminated soil, with increases of 27.0%, 45.6%, 25.5%, and 38.1% in fresh mass of shoot, dry mass of shoot, fresh mass of root, and dry mass of root compared to the Cd+EDDS treatment, and increases of 41.7%, 24.0%, 20.5%, and 19.5%, respectively, compared to the Cd treatment. Under different treatments, the Cd transport capacity of Bidens pilosa L. was weak, and the roots of the plants accumulated more Cd than the shoots. The combined treatment of 50 mg/kg NO₃⁻-N and EDDS significantly increased the enrichment of Cd by Bidens pilosa L. in Cd-contaminated soil, with increases of 45.8%, 59.5%, 46.1%, and 46.7% in Cd accumulation of shoot and root, bioconcentration factor (BCF), and phytoextraction efficiency (PEE) compared to the Cd+EDDS treatment, and increases of 125.7%, 181.1%, 125.5%, and 97.4%, respectively, compared to the Cd treatment. In addition, the effects of the three forms of nitrogen fertilizers combined with EDDS on the reactive oxygen metabolism of Bidens pilosa L. in Cd-contaminated soil varied. Among them, under the treatment of 50 mg/kg NO₃⁻-N combined with EDDS, peroxidase (POD) and superoxide dismutase (SOD) played a leading role in the antioxidant enzyme system. POD and SOD were increased by 8.8% and 36.9%, respectively, compared to the Cd+EDDS treatment, and 20.7% and 46.4%, respectively, compared to the Cd treatment; the contents of reduced glutathione (GSH) and proline (Pro) in plants increased by 12.7% and 86.3%, respectively, compared to the Cd+EDDS treatment, and increased by 7.3% and 70.7%, respectively, compared to the Cd treatment. Under this treatment, the plants alleviated the membrane lipid peroxidation damage caused by Cd stress by enhancing the activity of antioxidant enzymes and increasing the content of antioxidants, as a result, the level of hydrogen peroxide (H₂O₂) was significantly lower than that of Cd treatment and the tolerance of plants to Cd was improved. Considering all factors, the combination treatment of 50 mg/kg NO₃⁻-N and 1 mmol/L EDDS can improve the remediation efficiency of Bidens pilosa L. in Cd-contaminated soil. It is suggested that the combined remediation technology should be used to regularly plant and harvest Bidens pilosa L. with 67 days as a cycle in the growth period of this experiment, and the soil Cd content should be reduced by continuous cropping.