Abstract: Seedling transplanting with paper chain pots has been one of the most efficient and sustainable crop cultivations, in order to reduce the environmental impact, simple transplantation, and high seedling survival rate. The stronger seedling root system can be realized to promote multiple crop cultivation, land replanting index, and crop yield in high-yield and efficient agriculture, compared with traditional cultivation. However, it is still lacking in the equipment of paper chain pot, in order to fully meet the large-scale production in recent years. In this study, a booklet-making machine was developed to automatically fabricate the paper chain pots. Key processes were also integrated, such as gluing, indentation, paper feeding, and folding, in order to meet the agronomic needs of transplanting seedlings in various crops. A cost-effective solution was then provided for the production. Firstly, a prototype of a paper chain pot nursery booklet-making machine was designed, according to the hexagonal cross-section bowl chain seedling booklet. The motors and pneumatics were combined to drive the coordinated action of various working components. The formation and assembly of chain pots into booklets were realized to significantly reduce manual labor and production costs. Structural parameters were determined to optimize the key components. Among them, the glue coating device was used for the precise adhesive application; The indentation device was used to create the necessary indentation for folding and formation; The paper feeding device was to drive the movement of the paper through the machine; The folding paper collection device was assembled the chain pots into a booklet format. In addition, an operation control system was designed with various functions, such as one-touch start/stop, commissioning, cleaning, and operating instructions. An STM32 microcontroller was selected as the control core, in order to coordinate the movement of all components. As such, each device was optimized using the correct program during production. The working performance tests were carried out on the prototype of the machine using water-soluble adhesive, water-resistant adhesive, and nursery paper. The complete working test of making booklet results show that the average pass rate was 91.55% when the working efficiency was 60 copies/h and the chain bowl length was between 12.16 and 21.28 m. By contrast, the accuracy of paper feeding remained at a low level at different candidate job speeds, with a slip rate below 0.23%. The seedling experiment with the oilseed rape and tomato showed that the germination rate of seedlings with the made paper chain pot nursery booklet was 96.21%, indicating better seedlings growth. The paper chain pot nursery booklet can be easily dragged and unfolded into chains before transplanting, fully meeting the requirements of seedling cultivation and lightweight automatic transplantation. The performance indicators also met the requirements of the paper chain pot nursery booklet making, in order to effectively save manual labor and production costs for high efficiency. This research can provide new ways to realize mechanization and automation during transplanting.