应用超弱光子辐射评价菠菜叶片衰老方法可行性

习岗; 贺瑞瑞; 刘锴; 赵燕燕

doi:10.3969/j.issn.1002-6819.2014.17.034

应用超弱光子辐射评价菠菜叶片衰老方法可行性

西安理工大学理学院应用物理系，西安 710048

基金项目: 国家自然科学基金（51277151）；西安理工大学科技创新计划项目（2013CX019）

计量
- 文章访问数: 1854
- HTML全文浏览量: 0
- PDF下载量: 797
出版历程
- 收稿日期: 2014-04-25
- 修回日期: 2014-07-26
- 发布日期: 2014-08-31

Feasibility of evaluation method for spinach leaf senescence based on biological ultraweak photon emission

Department of Applied Physics, Institute of Science, Xian University of Technology, Xi'an 710048, China

摘要

摘要: 为了研究植物叶片衰老评价及其无损检测方法，采用外源H2O2诱发菠菜叶片衰老，研究了H2O2胁迫下菠菜叶片细胞超弱光子辐射的变化规律及其与叶片衰老的关系。结果表明，在H2O2胁迫过程中，菠菜叶片自发光子辐射随着胁迫时间的延长而增加，外界光诱导的延迟光子辐射也表现出明显的变化。根据细胞自发光子辐射和延迟光子辐射的生物学意义，采用延迟光子辐射积分强度和自发光子辐射的比值作为描述细胞整体状态的状态参量，结果发现，在H2O2胁迫过程中，基于细胞超弱光子辐射的菠菜叶片细胞状态参量的变化与叶绿素含量的变化呈现良好的正相关，相关系数为0.91437，暗示通过细胞状态参量的变化可以推测菠菜叶片细胞的状态与衰老程度，进而实现对菠菜叶片衰老程度的无损检测，为植物叶片衰老评价提供了一种新方法。
- 辐射 /
- 无损检测 /
- 叶绿素 /
- 叶片衰老评价 /
- H2O2胁迫 /
- 细胞超弱光子辐射 /
- 细胞状态参量
Abstract: Abstract: Evaluation of plant leaf senescence is one of the core issues of life science research, which is generally based on the physiological indicators such as chlorophyll content，malondialdehyde (MDA) content. This evaluation method requires to destroy plant cells，resulting in inaccurate measurements and many other issues. Non-destructive testing and evaluation of leaf senescence has not been resolved. Plant cells in life activity will produce ultraweak photon emissions which include spontaneous photon emission and delayed photon emission induced by external light. The ultraweak photon emission of living cells is closely related with many processes such as physiological metabolism, photosynthesis, and cell division. Through analysis and interpretation to the ultraweak photon emission of plant cells, a new method for non-destructive testing of plant leaf senescence could be proposed. For this purpose, spinach leaves in vitro were treated with H2O2. Spinach leaf senescence caused by H2O2 stress and the ultraweak photon emission of spinach leaf under H2O2 stress were studied in this paper. The results showed that the spontaneous photon emission of spinach leaves increased with the extension of H2O2 stress time and was positively correlated with changes of MDA content in leaf, with a correlation coefficient of 0.90415, and the changes of spontaneous photon emission in leaves reflected the changes of membrane lipid peroxidation degree in plant leaves. The study also found that the change of delayed photon emission induced by external light also occurred in the process of H2O2 stress. Compared with the control group, the delayed photon emission of spinach leaves decreased rapidly. According to the biological significance of spontaneous photon emission and the delayed photon emission of plant cells induced by external light, using the ratio of integrated intensity of the delayed photon emission to the spontaneous photon emission as a state parameter to describe the overall state of cells, it was found that the changes of cell state parameter based on ultraweak photon emission of cells were positively related to the changes of chlorophyll content in cells in the process of leaf senescence under H2O2 stress, with a correlation coefficient of 0.91437. This result suggested that the degree of cells senescence in leaf could be speculated through the change of cell state parameter based on ultraweak photon emission of cell, so as to realize non-destructive testing and evaluation about the degree of cells senescence of plant leaves. The photonics method of leaf senescence evaluation proposed in this paper needs to be verified in more experiments in different plant leaves.
- radiation /
- non-destructive examination /
- chlorophyll /
- evaluation of plant leaf senescence /
- H2O2 stress /
- ultraweak photon emission of cell /
- cell state parameter

HTML全文

参考文献(31)

[1]	梁秋霞，曹刚强，苏明杰，等. 植物叶片衰老研究进展[J]. 农学通报，2006，22(8)：282－285.Liang Qiuxia, Cao Gangqiang, Su Mingjie, et al. Research progress on plant leaf senescence[J]. Chinese Agricultural Science Bulletin, 2006, 22(8): 282－285. (in Chinese with English abstract)
[2]	Lim P O, Kim H J, Nam H G. Leaf senescence[J]. Annual Review of Plant Biology, 2007, 58(1): 115－136.
[3]	Tanaka R, Hirashima M, Satoh S, et al. The Arabidopsis- accelerated cell death gene ACD1 is involved in oxygenation of pheophorbide a: inhibition of the pheophorbide a oxygenase activity does not lead to the "stay-green" phenotype in Arabidopsis[J]. Plant and Cell Physiology, 2003, 4(12): 1266－1274.
[4]	Ren G, An K, Liao Y, et al. Identification of a novel chloroplast protein AtNYE1 regulating chlorophyll degradation during leaf senescence in Arabidopsis[J]. Plant Physiology, 2007, 144(3): 1429－1441.
[5]	Martin C, Thimann K V. The role of protein synthesis in the senescence of leaves[J]. Plant Physiology, 1972, 49(1): 64－71.
[6]	Hashimoto H, Kura H M, Katoh S, et al. Changes in protein content and in the structure and number of xhlorplasts during leaf senescence in rice seedling[J]. Plant and Cell Physiology, 1989, 30(5): 707－715.
[7]	Wang Y T, Yang C Y, Chen Y T, et al. Characterization of senescence-associated proteases in postharvest broccoli florets[J]. Plant Physiology and Biochemistry, 2004, 42(7/8): 663－670.
[8]	Abdelkhalik A F, Shishido R, Nomura K, et al. QTL- based analysis of leaf senescence in an indica/japonica hybrid in rice (Oryza sativa L)[J]. Theoretical and Applied Genetics, 2005, 110(7): 1226－1235.
[9]	孔汶汶，刘飞，方慧，等. 除草剂胁迫下大麦叶片丙二醛含量的光谱快速检测方法[J]. 农业工程学报，2012，28(2)：171－175.Kong Wenwen, Liu Fei, Fang Hui, et al. Rapid detection of malondialdehyde in herbicide-stressed barley leaves using spectroscopic techniques[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2012 , 28(2): 171 －175. (in Chinese with English abstract)
[10]	李伦，周湘萍，刘刚，等. 常绿树叶自然衰老的红外光谱研究[J]. 光谱学与光谱分析，2013，33(2)：340－343.Li Lun, Zhou Xiangping, Liu Gang, et al. Infrared spectroscopic study on leaf senescence of evergreen tree[J]. Spectroscopy and Spectral Analysis, 2013, 33(2): 340－343. (in Chinese with English abstract)
[11]	杜光源，唐燕，张嵩午，等. 小麦叶片衰老态势的核磁共振分析[J]. 农业机械学报，2014，45(4)：264－270.Du Guangyuan，Tang Yan，Zhang Songwu，et al. Investigation on senescence status of wheat leaf by nuclear magnetic resonance[J]. Transactions of the Chinese Society of Agricultural Machinery, 2014, 45(4): 264－270. (in Chinese with English abstract)
[12]	Mansfield J W. Biophoton distress flares signal the onset of the hypersensitive reaction[J]. Trends in Plant Science, 2005, 10(7): 307－309.
[13]	Yoon Y Z, Kim J, Lee B C. Changes in ultraweak photon emission and heart rate variability of epinephrine-injected rats[J]. General Physiology and Biophysics, 2005, 24(2): 147－159.
[14]	Yoshinaga N, Kato K, Kageyama C. Ultraweak photon emission from herbivory-injured maize Plants[J]. Naturwissenschaften, 2006, 93(1): 38－41.
[15]	Hidehiro I, Toshiyuki I, Wang G X, et al. Spontaneous ultraweak photon emission from rice (Oryza sativa L.) and paddy weeds treated with a sulfonylurea herbicide[J]. Pesticide Biochemistry and Physiology, 2007, 89(2): 158－162.
[16]	Yu Y, Popp F A, Sibylle S. Further analysis of delayed luminescence of plants[J]. Journal of Photochemistry and Photobiology B: Biology, 2005, 78(3): 235－244.
[17]	孙玉莹，毕京翠，赵志超，等. 作物叶片衰老研究进展[J]. 作物杂志，2013(4)：11－19.Sun Yuying, Bi Jingcui, Zhao Zhichao, et al. The advancement on leaf senescence[J]. Crops, 2013(4): 11－19. (in Chinese with English abstract)
[18]	Zimmermann P, Zentgraf U. The correlation between oxidative stress and leaf senescence during plant development[J]. Cellular & Molecular Biology Letters, 2005, 10(3): 515－534.
[19]	张海娜，谷俊涛，郭程瑾，等. 植物衰老的分子生物学基础[J]. 草业学报，2009，18(1)：163－170.Zhang Haina, Gu Juntao, Guo Chengjin, et al. The basis of molecular biology of senescence in plants[J]. Acta Prataculturae Sinica, 2009, 18(1): 163－170. (in Chinese with English abstract)
[20]	Hung K T, Hsu Y T, Kao C H. Hydrogen peroxide is involved in methyl jasmonate-induced senescence of rice leaves[J]. Physiologia Plantarum, 2006, 127(2): 293－303.
[21]	Chan Z L, Tian S P. Induction of H2O2 metabolizing enzymes and total protein synthesis by antagonistic yeast and salicylic acid in harvested sweet cherry fruit[J]. Postharvest Biology and Technology, 2006, 39(3): 314－320.
[22]	习岗，卢洪，李少华. H2O2诱导的植物叶片光子辐射的变化及意义[J]. 光子学报，2009，38(12)：3250－3255.Xi Gang, Lu Hong, Li Shaohua. Changes of biophoton emission of plant leaf induced by H2O2 and its significance[J]. Acta Photonica Sinica, 2009, 38(12): 3250－3255. (in Chinese with English abstract)
[23]	习岗，刘锴，张晓辉，等. UV-B诱导的大豆愈伤组织超弱光子辐射的动力学分析[J]. 光子学报，2010，39(8)：1449－1454.Xi Gang, Liu Kai, Zhang Xiaohui, et al. Dynamic analysis of ultra-weak photon radiation of soybean callus induced by UV-B radiation[J]. Acta Photonica Sinica, 2010, 39(8): 1449－1454. (in Chinese with English abstract)
[24]	刘萍，李明军. 植物生理学实验技术[M]. 北京：科学出版社，2007：39－42.
[25]	王学奎. 植物生理生化实验原理和技术[M]. 北京：高等教育出版社，2007：39－42.
[26]	王海波. 细胞状态观点的提出及其生命科学意义[J]. 科技导报，2008，26(4)：41－46.Wang Haibo. Concept of the cell state and its Significance in life science[J]. Science & Technology Review, 2008, 26(4): 41－46. (in Chinese with English abstract)
[27]	Marvin L S. Toxic oxygen species and protective systems of the chloroplast[J]. Physiologia Plantarum, 1988, 72(3): 681－689.
[28]	Rastogi A, Pospí?il P. Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells[J]. Plant Physiology and Biochemistry, 2010, 48(2/3): 117-123.
[29]	Rastogi A, Pospí?il P. Ultra-weak photon emission as a non-invasive tool for the measurement of oxidative stress induced by UVA radiation in Arabidopsis thaliana[J]. Journal of Photochemistry and Photobiology B: Biology, 2013, 125(5): 59－64.
[30]	Birtic S, Ksas B, Genty B, et al. Using spontaneous photon emission to image lipid oxidation patterns in plant tissues[J]. Plant Journal, 2011, 67(6): 1103－1115.
[31]	Setsuko Komatsu, Abu Hena Mostafa Kamal, Takahiro Makino, et al. Ultraweak photon emission and proteomics analyses in soybean under abiotic stress[J]. Biochimica et Biophysica Acta, 2014, 1844(7): 1208－1218.

施引文献

资源附件(0)

计量

文章访问数: 1854
HTML全文浏览量: 0
PDF下载量: 797
被引次数: 0

应用超弱光子辐射评价菠菜叶片衰老方法可行性

计量

出版历程

Feasibility of evaluation method for spinach leaf senescence based on biological ultraweak photon emission

计量

出版历程

目录