Abstract: Abstract: This paper explored the optimal substrate of wolfberry shoots for fermentation process and parameters. The study used an orthogonal experimental design with wolfberry shoots as the main raw material, dried chicken manure, cake, and urea as a nitrogen source, cellulose degrading bacteria, and EM complex composite bacteria and BM complex composite bacteria for the inoculation of exogenous microorganism. The effect of carbon and nitrogen ratio, microbial agents, nitrogen type, and nitrogen ratio on the fermentation process of wolfberry shoots for substrate production were studied. The parameters included composting temperature, carbon, nitrogen, bulk density, porosity, water holding capacity, air filled porosity, germination index, and C/N ratio. The results showed that adjusting the carbon and nitrogen ratio of 30:1, crude cellulose degrading bacteria inoculation, adding chicken or cake nitrogen, organic nitrogen: Inorganic nitrogen is 3:1 pile faster and longer duration of high temperature (above 50℃, respectively, 12, 13,12 and 12 d, more than 55℃, respectively reached 8, 9, 9 and 7d), accumulated a higher temperature, shortening the maturity time of the wolfberry shoots powder; carbon and nitrogen ratio, microbial agents, nitrogen type and nitrogen type had significant influence on total organic carbon and C/N value of a pile, carbon and nitrogen ratio, nitrogen type and nitrogen ratio had significant influence on total nitrogen and nitrate nitrogen, carbon and nitrogen ratio, microbial agents , carbon and nitrogen ratio, microbial agents, nitrogen type, and nitrogen ratio had no significant influence on ammonium nitrogen; Nitrogen type and nitrogen ratio of matrix on bulk density, total porosity, pore water retention, and aeration porosity had no significant influence, but the impact on the Chinese wolfberry shoots maturity matrix parameters were significant , carbon and nitrogen ratio, microbial agents , nitrogen type and nitrogen ratio of matrix effects on the seed germination index (GI) are important in this order: N ratio > ratio > microbial agents > nitrogen type. Taken together, the optimal substrate of wolfberry shoots for fermentation process and parameters carbon and nitrogen ratio of 30:1, inoculation crude fiber degrading bacteria inoculation, adding chicken or cake nitrogen, organic nitrogen: and inorganic nitrogen was 3:1. The research on how to change wolfberry shoots into a horticultural substrate through composting can not only solve the problem of an agricultural waste recycling point, but also supplement or replace non-renewable production of horticultural peat substrate, provide a source of raw materials, and reduce the cost of substrate cultivation, with some research value and practical significance.