Abstract: Water respiration and content of Algal,in eight ponds on the same outside condition, were measured and analysed in this research paper.Further more we designed the more accurate and more simply experiment method:ratio formula. After using mathematical statistic method to analyse the tropic of caparicorn of the experiment data,we obtained three groups of tunctional relations,which are the content of cholrophylla and speed of water respiration,the content of feofitin and speed of water respiration,the sum of chlorophyll and speed of water respiration,and then we studied their correlations.