加热法提取叶蛋白的工艺研究

    Extraction Technology of Leaf Protein by Heating Method

    • 摘要: 该文对加热法提取苜蓿叶蛋白的工艺进行了深入研究,以提高其蛋白质含量。分别进行了提取液加盐量和絮凝温度对蛋白质含量影响的单因素试验研究,还进行了不同加水、加盐量和pH值的提取液及不同絮凝温度对蛋白质含量影响的多因素试验研究,得到了提高蛋白质含量的可靠工艺条件。

       

      Abstract: Leaf protein (LP) is extracted from fresh leaves and stems. The resource of LP is very rich in China. LP production will loosen the short supply of feed protein.The paper studied the extraction technology of LP by heating method. The purpose was to find the suitable processing methods for increasing the protein content (PC) in the LP concentrate. The PC variation curve versus salt content at 0.1 %~0.6 % ,which is in the added water ,was obtained. The highest PC 58.8 % was gotten at salt content 0.2% . The test acquired a curve of the PC versus coagulative temperature (CT) at 60~90 ℃. It is that as higher CT as higher PC. The obvious increasing is located at CT at 60~70 ℃. PC increases 0.727 % with average increasing 1 ℃. The LP content increases slow down. It is only 0.0775 % ,when the temperature increases at 70~90 ℃. The orthogonal test on LP content versus added water, salt content, pH and CT was conducted. The decreasing order of the factors effecting PC was arranged by the R chart . That is salt content >added water>pH>CT. A potential curve was drown which show the four factors how effect the LP content. In order to reduce the energy consumption, a combined optimum processing condition is salt content 0.2 %, fresh plant∶water added =1∶2, pH 10, CT 70 ℃. It gain PC 63.76 %.

       

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