Abstract: Based on the test of electrostatic separation of Mallus spectabilis pollen,starting from pollen cell membrane potential and the charge of pollen in electrostatic field, in this paper the force and movement of pollen in an electrostatic field was analysed. The theory of electrostatic separation 2n pollen was studied and consequently an conclusion was drawn that the location of 2n pollen on the electrostatic field plate is lower than n pollen.