4320菌体蛋白饲料中双菌作用机制的研究

    Interaction Mechanism of Two Strains in Production of Microbiological Protein (MBP) 4320 for Feed Use

    • 摘要: 该文报导新发现的利用粗淀粉料及渣粕类原料不灭菌固体发酵生产4320菌体蛋白饲料时所用的两个菌株—优良黑曲霉选拔株No.303和白地霉菌As.2.361间的关系是一种偏利生关系,在利用这种关系进行混菌固体发酵生产4320菌体蛋白饲料时,发现有类似杂交现象的“菌丛”,通过试验,证明“菌丛”是这两个菌间偏利生关系的特殊表现。通过对偏利生作用因子的研究表明,No.303的菌株分泌的柠檬酸、酶等都是直接或间接或是综合作用的偏利生因子。特别是No.303分泌的柠檬酸及由蛋白酶分解的谷氨酸等能明显刺激As.2.361生长。通过正交试验得知,它们是两个最大的作用因子

       

      Abstract: This paper reports for the first time that the MBP fermentation is based on the relationship between the two selected good strains of G. Candidum As.2.361 and Aspergillus niger No.303, which is commensalism. The floras, like the appearance of hybridization formed by As. 2.361 when it is co cultivated with No.303 in 4320 MBP fermentation, are proved to be a specific feature of commensalism through Fusant Test, Heterocaryon Test, Substrate Mycelia Observation, Diffusion block Test and Microscopic Examination. The quantity of floras directly reflects the extent of commensalism and the quality of MBP 4320 as well, which is of great application value. Effective factors in commensalism are citric acid, protease, glucan amylase, and a amylase produced by No.303, and they function directly, indirectly and cooperatively. Among the factors, amino acids, especially Glu, catalyzed by protease from No.303 and citric acid produced by No.303 can stimulate growth of No.2.361 strikingly. They are the two newly found top commensal factors in producing MBP from starch materials, which is discovered through orthogonal test and is completely different from other reports that the main factors in producing SCP with starch materials are such enzymes as glucan amylase.

       

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