Abstract: The respiratory intensity and storage quality of post-harvest Red Globe and Kyoho grapes after being harvested at 0℃ and 0℃＋CT-2 were studied, and some physiological and biochemical indexes were determined regularly. Its objective was to discuss the storage property of Red Globe and Kyoho grapes under the storage condition with freshness keeping agent. The results showed that under the different conditions of storage, the respiratory pattern of whole cluster and berry of Red Globe and Kyoho grapes was non-climacteric, and that of stalk was climacteric. The respiratory intensity of Red Globe was lower than that of Kyoho. During the storage, the contents of reducing sugar, titratable acid, and vitamin C decreased gradually, among them, changes of titratable acid and vitamin C contents were negatively correlated with the time of storage significantly. Freshness keeping agent treatment inhibited obviously the respiration of grapes, lowered the loss of reducing sugar, titratable acid and vitamin C. Red Globe grapes had the longer storage life than Kyoho obviously. Under the condition of 0℃+CT-2+polyethylene film, the ratio of sound fruit of Kyoho was 87.8% after 135 days' storage; and that of Red Globe was 92.7% after 150 days' storage.