Abstract: In order to make good use of the maize pollen nourishment material, in the process of the maize pollen, a variable parameter ball mill which was made by ourselves was used to conduct a experiment on superfine grinding maize pollen. An orthogonal experimental design with four factors at two levels was adopted. A mathematical model describing effects of selected parameters on experiment criterion was developed. By means of optimal calculation a set of optimum parameters for the best superfine grinding of maize pollen was achieved with grinding drum rotation speed 476 r/min, grinding time 1.2 h, ratio of ball to maize pollen 7, and moisture content of the maize pollen below 5%. With those parameters, 100% of dilapidated walls for maize pollen were obtained and major diameter of maize pollen was below 8 micrometer, This research result laid a basic foundation for efficient utilization of maize pollen in food and cosmetic industry.