Abstract: Through artificial rainfall simulation experiment, this paper mainly studied the effect of applying the different concentration polymers including Polypropylene-acid, Polythene-hydri and Urea-formaldehyde resin. The results showed that macromolecule polymers applied on sloping land can put off time of producing runoff and decrease velocity of producing runoff. Compared with the control sloping, the runoff of sloping land applied macromolecule polymers was reduced 42.05%, and soil erosion was reduced over 58%. And also, through analyzing synthetically effect of them on soil and water loss, the appropriate concentration range of three macromolecule polymers were ascertained, Polypropylene-acid: 116～145 mL/m²; Polythene-hydri: 36～40 g/m²; Urea-formaldehyde resin: 180.0～240 mL/m² respectively, the polypropylene acid has the best effect in lowering surface runoff, reducing soil loss and increasing soil penetration. These three macromolecule polymers has the wide application foreground for soil and water conservation in the future, however their durative of effect and the optimal quantity need to be researched further.