Abstract: Grifola frondosa was cultured by fermentor. This research is expected to offer some academic bases for submerged culture technology in Grifola frondosa, and to establish material basis for exploitation of Grifola frondosa. The results showed that the optimal conditions of submerged culture ascertained were 25℃ of fermentation temperature, 6.5 of the beginning of pH value, 150 r/min of the rotating speed and 10% of putting in volume. In addition, pH value at 5.50～6.50 and the volume ratio of flow of air-compress at 1.00～1.40, the biomass of mycelium reached the highest level. By nutrition analysis, it is considered that the products of Grifola frondosa may be researched and exploited by submerged culture.