Abstract: Effects of species and concentration of total organic carbon(TOC) in the seawater on the accumulation of copper,lead and cadmium in visceral mass, muscle and gills of Paralichthys olivaceus when the copper,lead and cadmium concentrations were maintained at 0.5 mg/L, respectively were studied. It was demonstrated that when the species was same, the copper accumulation reduced significantly with the increase of TOC's concentration; when the concentration of TOC was same, Ulva pertusa excretion could reduce the copper accumulation more than Paralichthys olivaceus. It was demonstrated that all the excretions of Ulva pertusa and Paralichthys olivaceus complexed with copper,lead and cadmium, respectively were not well available for fish uptake; the species and concentration of TOC in the seawater could not affect the accumulation order in the tissues of Paralichthys olivaceus, the accumulation order of heavy mentals in the fish tissues was visceral mass>gills>muscle. The accumulation order of three heavy metals in the tissues of visceral mass and muscle was Cu>Pb>Cd; The accumulation order of three heavy metals in the tissues of gills was Pb>Cu>Cd.