Abstract: The study on the solution and attenuation properties of ozone in water and the lethal effect of ozone water on Alicyclobacillus on apple surface was conducted. The emphases were put on sterilizing tests by using blowing ozone water and ozonized water of different concentrations. The results were validated by simulated sterilizing using Alicyclobacillus inoculated apples. The results showed that ozone water with concentration of 31 mg/L could kill 99.96% of Alicyclobacillus in 15 min, and sterilizing effect was better by using blowing ozone water than by using ozonized water.