Abstract: In this paper, an artificial neural network model was used to assess sustainable utilization of regional water resources. First, stochastic method was used to form enough assessment indexes sequence. Second, by using back-propagation networks, assessment indexes sequences and their assessment grade values were considered as input layer units and output layer units to deveiop artificial neural network assessment model. Finally, the model was applied to assess sustainable utilization of water resources in Hanzhong basin and Huaihe watershed in China. The assessment results showed that the model was practical and convenient to use. It is feasible to use the model to assess the sustainable utilization of regional water resources.