Abstract: The content of total flavonoids in sesame leaves and its extraction technology were studied in this paper. By means of the methods of ethanol-extracting and ethanol-extracting with microwave processing, the optimum extraction conditions of flavonoids in sesame leaves were determined. The results showed that the content of total flavonoids in sesame leaves was 0.98%, the optimum conditions of ethanol-extracting were obtained as follows: 80% ethanol as extraction solvent at 80℃ for 2.5 h, and the ratio of material to solvent was 1∶25. Using the technological parameters above mentioned, the average extraction rate of total flavonoids in sesame leaves was 95.6%. Meanwhile, the optimum conditions of ethanol-extracting with microwave processing were as follows: 200 W of microwave power for 70 s, 80% ethanol extracting at 80℃ for 30 min, and the ratio of material to solvent was 1∶25. Using this technology, the extraction rate of total flavonoids in sesame leaves was 95.8%.