Abstract: A technology for extracting diosgenin from fenugreek seeds was studied by using enzymatic hydrolysis and water extraction methods. To take the extraction ratio of diosgenin as an index, the optimal enzymolysis conditions for improving the yield of diosgenin were investigated. The conclusion can be drawn as follows: compared with the simple water extraction methods, the enzyme treatment was beneficial to increasing yield of diosgenin; and the optimum enzymolysis parameters are: adding 14 times volume of water as much as that of sample and adding cellulase 10U per gram degreased fenugreek seeds, hydrolyzing for 6 h at 45℃(pH 4.6). The further water-extracting results indicate that the extraction ratio of diosgenin could be increased by 9.1%, when the powdered fenugreek seed was extracted for 70 min at 90～100℃ after being enzyme-hydrolyzed.