鲤鱼精蛋白的提取与抗菌稳定性研究

Extraction of protamine from carp spermary and its antibacterial stability

摘要: 该文研究了采用超声波技术提取鲤鱼抗菌精蛋白的工艺条件和抗菌稳定性。实验结果表明：采用超声波技术提取鱼精蛋白硫酸用量少，提取时间短。用1.0A强度的超声波和5%的硫酸溶液提取鱼精蛋白，提取时间20 min，能得到较高活性的鲤鱼抗菌精蛋白。并进一步研究了pH值、温度、金属离子和蛋白酶对鲤鱼精蛋白抗菌活性的影响。

Abstract: The extraction of antibacterial protamine from carp spermary by ultrasonic technology was studied. The results show that H₂SO₄ concentration is low, and extracting time is also short by using ultrasonic technology. Protamine of higher antibacterial activity could be extracted with 1.0 A ultrasound, 5% H₂SO₄ for 20 min. And the effects of pH values, temperature, metal ion, protease on protamine antibacterial activity were also investigated.