Abstract: There are multi-model threading machines in market at present, but in order to reduce the cost of manufactured goods, the parameters of machine are designed by experience only, therefore, the problems of larger power consumption, lower efficiency, worse threading quality are emerging. Through the method of orthogonal experiments and computer statistic analysis software, the factors affacting the threading performance target of a new 9JST-20 type of stalk threading and mixing machine were studied, so as to provide the theoretical foundation for the development and production of the stalk threading machine. Through the experimental study, the main and subordinate factors and the influence rule on the threading target were obtained.