Abstract: The extraction conditions of antioxidative substances from black rice pericarp were studied by orthorhombic analysis and total antioxidant capacity (TAC) was used as the activity monitoring parameter. The optimized extraction conditions were as follows: using 60% ethanol as the solvent to extract antioxidative substances from black rice pericarp at 60℃ for 4 h, and with a material/solvent ratio of 1∶4. The one-time extraction rate under such conditions was 71.38%, and the twice extraction rate was over 85%. In order to remove the fat, the solvent of petroleum ether or normal hexane, which did not have significant effects on TAC of the extract, could be used as the degreaser. By comparing static and dynamic absorption performance, macropore adsorptive resin NKA-Ⅱ that had the highest performance in absorbing antioxidative extract from black rice pericarp and optimum absorption relieving agent 70% ethanol solution were selected. After absorption and separation by NKA-Ⅱ, the TAC and total anthocyanin content of antioxidative extract from black rice were increased by 4.00 and 4.01 times, respectively.