Abstract: To improve the concentration of flavonoids compounds in the water-extraction solution of fresh loquat leaves, optimal processing conditions were investigated by using the factors and uniform design. The results showed that when the size of cut leaves was 1.2 mm×1.3 mm and the solid/liquid ratio was 34 g/250 mL, the optimal processing parameters were pH 8.0, temperature 100℃ and extraction time 105 min. Under the rational conditions, the concentration of flavonoids in the extract was 0.979 mg/mL.