条斑紫菜多糖超声提取及其对U937细胞生长抑制影响的研究
Ultrasonic extraction of polysaccharides from Porphyra yezoensis and their inhibition effects on U937 cell growth
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摘要: 采用超声辅助提取技术进行条斑紫菜多糖的提取，并进行柱层析纯化。经正交试验确定的条斑紫菜粗多糖提取的最佳工艺条件为：15 g干紫菜粉加入2 L水，提取液循环转速为12 r/s，提取液温度为40℃，超声功率为800 W，超声处理时间/超声间隙为3 s/2 s和超声全程时间为2 h，对应的得率为26.29%。粗多糖分别经50%、60%、70%和80%乙醇分步醇沉，沉淀出四种不同级分的半纯品，用DEAE-52纤维素和Sephadex G-200将其中两种多糖半纯品纯化，分别得到5个和3个不同分子量的级分，分别考察了这两种紫菜多糖半纯品及各自分级产物对人血癌细胞U937细胞的体外生长抑制的抗肿瘤试验，结果前者及其分级产物对U937细胞生长有一定的抑制作用，后者及其分级产物则有一定的促进作用。Abstract: Polysaccharides were extracted from Porphyra yezoensis using ultrasonic extraction method and were purified the polysaccharides with DEAE-52 and Sephadex G-200 chromatogram. Through orthogonal tests,we achieved the best conditions of ultrasonic extraction process as follows: weight of Porphyra yezoensis is 15 g, volume of water is 2 L, rotation speed of pump is 12 r/s, extraction temperature is 40℃, ultrasonic power is 800W, time ratio of ultrasonic processing and intermission is 3s/2s and ultrasonic processing time is 2 h. In this method, production rate of rough polysaccharides reaches 26.29%. Then we precipitated rough polysaccharides in order with 50%, 60%, 70% and 80%(V/V) ethanol and got four ingredients of rough polysaccharides. These ingredients were purified with DEAE-52 and Sephadex G-200 chromatogram and then 5 or 3 kinds of different molecular weight polysaccharides were obtained, respectively. The former parts have a little inhibition effects on U937 cell growth, yet the latter parts have promotion effects.
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Keywords:
- polysaccharide /
- ultrasonic extraction /
- Porphyra yezoensis /
- U937 cell
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