Abstract: A new method for isolation and purification of mogrosides with macroporous resin was studied. The results showed that the optimum column isolation condition was as follows: the aqueous solution of crude extract of momordica grosvenori.(MGC) at pH 9.0 was loaded onto an D101 resin column, and then washed with pH 9.0 aqueous solution, distilled water, 30% ethanol and 60% ethanol in proper order at 40℃. The eluent of 60% ethanol was collected, concentrated and freeze-dried, the mogroside extract (MGI)was obtained and the contents of its main mogrosides were all more than that of MGC, especially the content of its mogroside V was 69.24% (HPLC), increasing by 41.12% over MGC.