Abstract: The living marine fish transportation device designed to tackle survival rate of fish in long time/distance transportation affected by three major facors, including water temperature,D.O.and water quality.The device can be installed to cargo trucks. It has such functions as cooling system,oxygen increasing,bacteria killing,seawater circulating and purifying and electric power supply. It took seven months to test the device by transporting various marine fish totaling 48 tons 33 times. The results indicated that under the ratio of fish to water as 1∶6 and the transporting duration of 62 h, the survival rates of marine fish averaged over 95%. Its use, in transporting rare and valuable marine fish can result in better economic benefits.