Abstract: As, Pb were added to study the speciation distributions and the characteristic of chemical, biological availability in acid, neutral and alkali purple soil. The results showed that the speciation distribution varied from three purple soils and the differences also existed in the soil between treatments of the primitive samples and the As, Pb added samples. The experiments were conducted to compare the effects of some extraction-reagents in terms of the extraction ability and the living-culture usefulness. The results indicate that the extraction methods by 0.5 mol/L NaHCO₃ for As, 2.5% HAc for Pb should be adopted in three purple soils. The As, Pb contents absorbed in plants were well related to its chemical availability in the soil but the absorbed speciation varied from different soils. The experiment also showed that H₃BO₃ could be used to restrain the F-interference in the operation of As speciation separations.