Abstract: The vermicompost-based medium at different fertilizer applying levels (CO(NH₂)₂, 0.2～0.8 kg·m^-3; KNO₃, 0.2～0.8 kg·m^-3) was used to produce eggplant plug seedlings. The results showed that fertilizer addition distinctly elevated the electric conductivity of media, increased the chlorophyll content and net photosynthetic rate in seedling leaves, and significantly enhanced the growth and development of plug seedlings. The optimal amount of fertilizer addition was selected to be 0.2 kg·m^-3 of CO(NH₂)₂ and 0.4 kg·m^-3 of KNO₃. This experiment will provide the scientific fertilizer applying bases for producing the healthy eggplant plug seedling using the vermicompost-based media.