Abstract: The experiment was carried out to study the effects of fertilization levels ［no fertilization(CK), sole organic fertilization(SOF), theoretical fertilization(TF), conventional fertilization(CF), and overabundance fertilization(OF)］ on the growth and development of tomatoes and the results were as follows: the plant height, stem diameter, leaf number and leaf area of the tomato increased with the increase of fertilization levels. The different fertilization levels have no effect on SLA, LAR, ADAN and single fruit weight. High fertilization level causes the obvious decrease of LNFI, and it also causes the increase of FNFI, fruit and biologic yield. Yield taxis is CK3-N in the tomato were raised, the content of soluble sugar and the ratio of soluble sugar in the tomato decrease and the quality and flavor of the tomato become bad. Although high N level in greenhouse plantation causes the high yield of tomatoes temporarily, it violates the principle of sustainable production and high quality of fruit in greenhouse.