Abstract: The experiment was carried out to study the effects of AMF inoculated different substance (organic soil, the mixture of vermiculite and peat, soil from field) on growth of tomato and activity of PAL and PPO. The results showed that there were remarkable effects of improving growth on height and width of stem; the mixture of vermiculite and peat was the best to growth of tomato and dry matter increased by 131% compared with the control; different substances affected infection and growth of AMF, and root colonization in organic soil was the highest; for 35, 50, 65 days after inoculation, PAL, PPO showed the trend from high to low, and between different substrates there existed significant differences.