Abstract: Effects of different picking days and storage days on the quality of gynoecious inbred line of mini-cucumber seeds were studied. The results showed that the best treatments were 40 days after picking, 5 days storage and 45 days after picking, 10 days storage, which reached the largest data on the one-thousand-grain weight, germination percentages, germination index, average fresh seedling weight and vigor index of Cucumis sativus seeds. While data of the treatment on 35 days after picking and 20 days storage were the smallest.