氧化还原类酶活性在农业废弃物静态高温堆腐过程中变化的研究

    Changes of oxidization and reduction enzymes of agricultural waste materials during composting at high temperature and static state

    • 摘要: 以鸡粪和麦秸为原料,在静态通气条件下,研究了堆腐过程堆体温度及氧化还原酶活性变化。结果表明,添加微生物菌剂后,堆体不同部位温度均高于CK(不加微生物菌剂)处理,且升温阶段持续时间较短。微生物菌剂处理堆料50℃以上的持续时间为14~20 d,CK处理50℃以上的持续时间7.5~10 d;添加微生物菌剂处理的过氧化氢酶活性在第1~26 d均大于CK处理;堆料中添加菌剂处理在堆腐中期的脱氢酶活性大于CK处理;添加菌剂处理在堆肥的第5~28 d,多酚氧化酶活性大于CK处理的活性;说明微生物菌剂可促进有机物的降解及其降解产物的转化。添加菌剂处理在堆腐30 d后E4/E6(为胡敏酸在465 nm与665 nm波长下吸光值的比值)比值为1.57~1.68,CK处理为2.16~2.41,表明添加菌剂能促进腐殖质的缩合和芳构化。

       

      Abstract: An experiment was conducted with mixed materials of chicken excrement and wheat straw to study the changes of temperature and oxidization and reduction enzyme during rottening processing in a static state. Results showed that temperatures of different positions of the treatments of adding microorganism fungus were higher than those of CK, and the temperatures rose more quickly than those of CK. The temperature of the materials adding microorganism fungus above 50℃ lasted for 14~20 d, and that of CK was 7.5~10 d. The activity of enzyme of H2O2 in the materials adding microorganism fungus was higher than that of CK during 1~26 d, and dehydrogenase activity was higher than that of CK in the middle period of the composting. All of them indicated that the microorganism fungus could promote the decomposition. During 5~28 d the materials of adding microorganism fungus, the polyphenol oxidized activity was higher than that of CK, and the E4/E6 values of materials of adding microorganism fungus were 1.57~1.68, and the E4/E6 values of CK were 2.16~2.41 after 30 d, which indicated that microorganism fungus could promote processing of humus.

       

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