Abstract: A method for separation and purification of flavonoids from shaddock skin with macroporous resin was studied, with the content and recovery rate of flavonoids as indexes. The results are as flollows: among six types of macroporous adsorption resin choosed, AB-8 type macroporous resin is the best for separating and purificating shaddock skin flavonoids; the optimun conditions is: the volume of the post is 160 mL, the loaded amount of shaddock shin extract is 62.5 mg/mL, wash with water(pH 4.0) and then use 30% ethanol as eluant. The AB-8 type resin can be used 3 times repeatedly. The content of flavonoids in the 30% ethanol eluant is 39.67% with the recovery rate of 62.48% after purification with AB-8 type resin. High performance liquid chromatography(HPLC) analysis result show that the obtained flavonoids is mainly flavone glycoside.