Abstract: The technology for protein extraction from defatted corn germ with thin alkaline solvent was studied. Mathematical model for predicting the extraction rate of protein was set up by central composite design and response surface analysis (RSA), and the extraction technology was optimized. The results show that optimum conditions of extraction are: ratio of water to material of 11 mL/g, pH value of 8.7, the temperature of 46℃. The rate of extraction reached 58.46% at above-mentioned conditions for 90 min.