Abstract: Rice protein was hydrolyzed by proteolytic enzymes for preparing rice peptide. The experiment was conducted to investigate the hydrolysis of rice protein by alcalase, nectruse, protamex and flavourzyme. Results showed that alcalase was the most effective protease. The appropriate hydrolysis conditions for alcalase are as follows: the concentration of rice protein is 10%, pH 9.0, 45℃, the ratio of alcalase to rice protein is 48 AU/kg, 150 min. Under these conditions, 46.8%(w/w) of rice peptides with 71.3% purity from total rice protein could be prepared. Rice peptides have better solubility and lower viscosity that can be extensively used in the food production.